摘要
采用SRAP分子标记技术,对不同产地的41份白三叶材料进行遗传多样性分析,筛选出20对随机引物组合,获得以下结果:1)20对随机引物组合共扩增出479条清晰可辨的条带,每对引物组合PCR扩增出13～44条带纹,平均为23.95条,多态性条带411条,多态性位点百分率(PPB)占85.16%,多态性信息含量(PIC)范围为0.182～0.334,平均为0.268,表明供试白三叶种质具有丰富的遗传多样性;2)材料间遗传相似系数(GS)范围为0.5866～0.9896,平均GS值为0.7307;表明供试白三叶种质在分子水平具有相对较远的亲缘关系;3)对所有材料进行聚类分析和主成分分析发现,在GS=0.71的水平上,可将41份白三叶材料分成3大类,大部分来自相同或相似生态地理环境的材料能聚为一类,呈现出一定的生态地理环境分布规律。
SRAP（sequence-related amplified polymorphism）markers were used to analyze genetic diversity of 41 white clover（Trifolium repens）accessions using 20 pairs of random primers.The results were：1）The primer pairs amplified 479clear bands,including 411 polymorphic bands,which ranged from 13 to 44 bands per primer pair with an average of 23.95 bands.The percent of polymorphic loci was 85.16%,and the polymorphism information content ranged from 0.182to 0.334 with an average of 0.268,which showed that the tested white clover had a rich genetic diversity of germplasm.2）Genetic similarity ranged from 0.586 6to 0.989 6 among all accessions with an average of 0.730 7,which also suggested that there was a rich genetic variation among the white clover germplasms.3）Cluster analysis and principal component analysis of 41white clover accessions divided them into 3 groups on the genetic similarity of 0.71 and most of the accessions from the same or similar ecologic geographical environment clustered in one group,showing a certain degree of eco-geographical distribution.
出处
《草业学报》
CSCD
北大核心
2010年第5期130-138,共9页
Acta Prataculturae Sinica
基金
国家科技支撑计划(2008BADB3B10)
四川农业大学青年创新基金(002325)资助
关键词
白三叶
SRAP
遗传多样性
white clover（Trifolium repens）
SRAP
genetic diversity
