摘要
用来自大麦 7个连锁群不同位置的 35个SSR标记研究了西藏不同地区的 5 0份野生二棱大麦 (H .spontaneum )的遗传多样性及地理分化。结果表明 :SSR的多态性高 ,其多态性DNA片段比例达 97.4 4% ;每个SSR位点检测到 1～ 14个等位变异 (4 .0 4个 /位点 ) ;西藏山南地区的遗传多样性和等位变异数最高 ,分别为 0 .4 933和 3.35。变异的 1.0 8%～ 37.5 4% (平均为 13.0 9% )是由于地区的差异引起 ,表现出一定程度的地理分化。最后 ,讨论了SSR引物的选择及SSR标记的可靠性和西藏野生大麦的起源问题。推测西藏山南地区可能是西藏野生二棱大麦的起源中心 ,也是西藏野生大麦和中国栽培大麦的起源中心。
This paper studied genetic diversity and geographical differentiation of 50 accessions of the two rowed wild barley H. spontaneum from different regions of Tibet using 35 SSR markers from different locations of 7 barley linkage groups. The results showed that SSRs had high polymorphism, 1～14 alleles of each SSR locus were detected with an average of 4.04 alleles/locus; The highest averages of both genetic diversity and alleles (0.4933 and 3.35, respectively) were all occurred in Shannan region of Tibet. 1.08%～37.54% of total variation with the average 13.09% was produced by geographical differentiation. Finally, the issues on selection of SSR primers and reliability of SSR markers as well as origin of Tibetan wild barley was discussed. Shannan region in Tibet could be inferred to be a center of origin on Tibetan two rowed wild barley, and also on Tibetan wild barlay, and on cultivated barley in China.
出处
《高技术通讯》
EI
CAS
CSCD
2003年第10期46-53,共8页
Chinese High Technology Letters
基金
中国科学院"百人计划"资助项目
关键词
西藏
野生二棱大麦
遗传多样性
DNA
中国
青藏高原
微卫星
SSR
Tibet, Wild barley, Simple sequence repeats, Genetic diversity, Origin, Geographical differentiation
