摘要
建立了香花槐组培苗快繁无性系.在改良MS培养基中添加ρ(6.BA)/mg L-1=0.35-0.5、ρ(NAA)/mg L-1=0.05-0.08,ρ(GA3)/mg L-1=0.07-0.1作为生长培养基.在丛生芽诱导培养基中,ρ(6-BA)/mg L-1=0.8-1.4,其余成分同生长培养基.两种培养方法同时使用,保证了组培苗繁殖系数为5左右.生根培养基中大量元素为MS基本培养基的1/4,ρ(IBA)/mg L-1=0.1-0.5、ρ(IAA)/mg L-1=1.7-2.5,香花槐组培苗的生根率为90％.炼苗后组培苗的移栽成活率为85％,且植株表型未见变异.将MS基本培养基中硝酸钾的含量由1.9g/L提高至2.199-2.931g/L,可满足每代香花槐试管苗生长25-35 d期间对钾的需求.将培养基中6-BA的含量降至0.4 mg/L,并采用合适的组培容器,在连续培养2、3代后,可将超度含水态苗的发生频率控制在10％以下.在6 mo内生产100万株香花槐组培移栽苗.
Clonal propagation of Robinia cv. Idaho plantlets was established. MS medium with ρ(6-BA)/mg L-1=0. 35-0.5,ρ(NAA)/mg L-1=0.05-0.08 and ρ(GA,)/mg L-1=0.07-0. 1 was used for its growth medium. In inducing medium,ρ(6-BA)/mg L-1 =0. 8-1.4 was used for inducing cluster buds. Two media were used so as to keep regenerating ratio of plantlets about 5. Each of more than 90% plantlets regenerated 2-4 roots when they were cultured in the 1/4 MS medium with ρ(IBA)/mg L-1 =0.1-0.5 and ρ( IAA)/mg L-1=1.7-2.5. Over 85% of them survived after transplanted in greenhouse, and no obvious variation in phenotype was observed. The content of KNO3 in the MS medium increased from 1.9 g/L up to 2. 199-2. 931 g/L to meet the need of plantlets that had been growing in medium for 25-35 days. The ratio of hyperhydric plantlets was reduced to 10% by substracting the content of 6-BA to 0.4 mg/L in medium and by using the containers which could exchange air easily. And one million plantlets were produced in only 6 months. Fig 1 , Tab 1 , Ref 16
出处
《应用与环境生物学报》
CAS
CSCD
2004年第2期162-165,共4页
Chinese Journal of Applied and Environmental Biology
基金
东北师大林木生物工程有限责任公司资助~~
关键词
香花槐
组织培养
快速繁殖
育苗
MS培养基
Robinia cv. Idaho
tissue culture
large-scale propagation
hyperhydricity
