摘要
目的:建立照山白药材中金丝桃苷的含量测定方法。方法:样品用90%的乙醇回流提取,提取液滤过,以RP-HPLC法测定,用DiamonsilTMC18色谱柱(250 mm×4.6 mm,5μm),以甲醇-5%磷酸-三乙胺(45:55:0.36)为流动相,流速为1.0mL·min-1,检测波长为360nm。结果:金丝桃苷的保留时间约为15 min,且与其它峰的分离度大于1.5。金丝桃苷的线性范围为0.12-0.28μg(r=0.9999),最低检测限为2.9 ng,定量限为0.029μg,平均回收率和RSD分别为99.8%和0.90%。结论:该方法简便快速,结果准确可靠,可为照山白药材的质量评价提供有效手段。
Objective: To establish the method to determine the concentration of hyperin in Folium Rhododendri Mi cranthi. Method:The sample was extracted with ethanol of 90% under reflux,then the extract solution was filtered. RP - HPLC was used for the determination of hyperin in Folium Rhadodendri Micranthi. The chromatographic procedure was carried out using Diamonsil TM18(250 mm×4. 6 mm,5μm) as an analytic column and a mixture of 45 volume of methanol,55 volume of 5% of phosphoric acid,0. 36 volume of triethylamine as a mobile phase at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 360 nm. Results:The peak of hyperin appeared on a-bout 15 minutes. Resolution of hyperin was more than 1. 5 and its linear range was between 0. 12 and 0. 28μg(r = 0. 9999). The minimum limit of detection was 2. 9 ng and the limit of quantitation was 0. 029μg. The average recovery and RSD value of hyperin were 99. 8% and 0. 90% ,respectively. Conclusion:This method is sensitive and quick and can be used for the quality control of Folium Rhododendri Micranthi.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2004年第2期162-164,共3页
Chinese Journal of Pharmaceutical Analysis
