摘要
目的:建立高效毛细管电泳法测定地塞米松磷酸钠脂质体中药物含量。方法:毛细管(60 cm×75μm,有效长度53cm);运行缓冲液100 mmol·L-1硼砂(0.1 mol·L-1盐酸调pH 9.2),高压进样5 s,分离电压20 kV,温度为20℃,头孢拉定为内标,检测波长为240 nm。结果:地塞米松磷酸钠在0.78-50 μg·mL-1浓度范围内线性关系良好(r=0.9998),地塞米松磷酸钠的平均回收率为98.5%。结论:本方法准确、简单、快捷,适用于地塞米松磷酸钠脂质体的质量控制。
Objective:To establish an HPCE method for the determination of dexamethasone sodium phosphate in liposome. Methods:HPCE was used. The best condition was achieved with a fused silica capillary tube(60 cm ×75μm,effective length of 53 cm) ,100 mmol·L-1 sodium tetraborate decahydrate buffer(pH 9. 2 adjusted by 0. 1 mol
·L-1 hydrochloric acid) ,at a constant voltage of 20 kV and temperature at 20℃Cefradine was used as internal standard. The UV detection wavelength was 240 nm. Results:The calibration curve was linear in the range of 0. 78
- 50μg·mL-1 for dexamethasone sodium phosphate(r= 0. 9998). The average recovery of dexamethasone sodium phosphate was 98. 5%. Conclusion:This method is simple, quick, accurate and the assay result is suitable for the quality control for dexamethasone sodium phosphate in liposome.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2004年第2期117-119,共3页
Chinese Journal of Pharmaceutical Analysis
基金
广东省自然科学基金(020056)
广东省医学科研基金(A2002371)资助
