摘要
目的 :揭示羊栖菜多糖 (SFPS)的抗肿瘤作用机制。方法 :采用流式细胞仪观察羊栖菜多糖对肿瘤细胞周期及细胞凋亡的影响。采用Fluo 3 /AM探针标记 ,激光共聚焦技术观测细胞内 [Ca2 + ]i。结果 :羊栖菜多糖可阻滞SGC 790 1人胃癌细胞由G0 /G1期进入S期 ,升高细胞凋亡指数 (APO % )。可使SGC 790 1细胞内 [Ca2 + ]i先升高然后下降 ,给CaCl2 后 ,[Ca2 + ]i又升高。结论 :羊栖菜多糖通过升高肿瘤细胞内 [Ca2 + ]i启动肿瘤细胞凋亡机制而达到抗肿瘤的作用 ,[Ca2 + ]i升高时Ca2 + 来源于细胞内钙库释放。
Objective: To study mechanism of antitumor a ctivity of Sargassum Fusiforme Polysaccharide(SFPS). Method: The effect on cell cycle and apoptosis was studied with flow cytometry(FCM). Intracellular calcium concentration [Ca 2+ ]i was marked with Fluo-3/AM and measured with laser scanner confocal microscope(LSCM). Result: SFPS inhibited G 0/G 1 stage SGC-7901 from entering to S stage an d increased APO%. The [Ca 2+ ]i showed a transient rise and return to th e original level. The concentration could be raised again by administering CaCl 2. Conclusion:The antitumor effect of SFPS seems to be accomplished through the apoptosis associated with the increase in intracellular calcium concentration. Intracellular stores release the calcium during its acti on.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2004年第3期245-247,共3页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目 ( 30 2 71 598)
教育部科技研究重点项目 ( 0 1 0 35)
黑龙江省自然科学基金项目 (D0 0 1 8)
黑龙江省普通高等学校骨干教师创新能力资助计划资助项目 ( 2 0 0 1 0 1 5)
