摘要
目的 观察羊栖菜多糖对6种不同组织肿瘤的抑瘤作用,并对其作用机制进行了讨论。方法 通过对S180、H22荷瘤小鼠瘤重和生存时间的研究,观察了羊栖菜多糖的体内抗肿瘤作用,采用MTT法和集落形成实验法观察羊栖菜多糖的体外抗肿瘤作用。采用流式细胞仪观察羊栖菜多糖对肿瘤细胞周期及细胞凋亡的影响。结果 羊栖菜多糖对人胃癌细胞SGC-7901和人直肠癌COLO-205有较好的疗效。羊栖菜多糖可阻滞SGC-7901人胃癌细胞内G0／G1期进入S期,升高细胞凋亡指数(APO％)。结论 羊栖菜多糖对人胃癌细胞和直肠癌细胞效果较好,这一作用是通过诱导肿瘤细胞凋亡达到的。
Aim To investigate the antitumor effect of polysaccharide from Sargassum fasiforme on six kinds of tumor cell strains and the mechanism of antitumor activities was discussed at the same time. Methods The anti-tumor activities were observed in vivo by testing the tumor weight of S180 mice and survive time of H22 mice. MTT and Colony-forming methods were applied to study the anti-tumor activities of SFPS in vitro. Influences on cell cycle and apoptosis of SFPS were observed by FCM. Results SFPS show potent anti-tumor activities to SGC-7901 and COLO-205. It could prevent the transforming of SCG-7901 from G0/G1 to S phase and increase the apoptosis index (APO%). Conclusion SFPS shows potent anti-tumor activities to SGC-7901 and COLO-205 by inducing the apoptosis of tumor cells.
出处
《中国海洋药物》
CAS
CSCD
2004年第4期7-10,共4页
Chinese Journal of Marine Drugs
基金
国家自然科学基金资助项目(30271598)教育部科技研究重点项目(01035)黑龙江省自然科学基金(D00-18)黑龙江普通高等学校骨干教师创新能力资助计划资助项目(2001015)。
关键词
羊栖菜多糖
SGC-7901人胃癌细胞株
细胞凋亡
抗肿瘤
<Keyword>Sargassum fusiforme polysaccharide (SFPS)
human gastric cancer cell
apoptosis
anti-tumor activities
