摘要
为了选育高产食品蛋白酶菌株并研究其最佳发酵条件 ,从豆腐乳中分离出总状毛霉MR137,用CO2 激光辐射诱变。激光功率 10W ,扩束光斑直径 0 .4cm ,照射距离 2 2cm ,照射时间 4 0s。经初筛、复筛 ,选育出高产蛋白酶变异株 (MR137 3)。经传代培养 ,MR137 3具有稳定的高产蛋白酶活性。对MR137 3的固体发酵条件进行研究发现 ,培养温度 30℃ ,培养时间 72h ,培养基pH6 .0 ,培养基含水量 6 5 % ,是MR137
Mucor racemosus 137 was isolated from fermented bean curd and brought to mutation by radiation of CO 2 laser. The duration for radiation was 40s with distance of 22cm, and the diameter of laser beam was 0.4cm. High proteinase-producing mutant MR137-3 was obtained after screening cultures. The MR137-3 was found to have stable proteinase-producing activity in descendant cultures. The optimal culturing could be obtained at 30℃, pH6.0, culturing duration of 72h, with water content of 65% of culture medium.
出处
《激光技术》
CAS
CSCD
北大核心
2004年第2期128-130,共3页
Laser Technology
关键词
CO2激光
总状毛霉
发酵
蛋白酶
优化条件
CO 2 laser
mucor racemosus
fermentation
proteinase
optimination condition
