摘要
实验发现 ,在 p H8.8的 H3BO3- Na OH介质中 ,镍 ( ) -血清蛋白配合物在单扫示波极谱上 ,在- 0 .6 0 V处 (vs.SCE)会产生吸附性质的催化前波 ,该波二阶导数波高与蛋白质浓度在一定范围内呈线性关系 ,对于牛血清蛋白 (BSA) ,线性范围为 1～ 2 9μg/ m L,检出限为 0 .5 μg/ m L;对于人血清蛋白 (HSA) ,线性范围为 0 .8～2 6 μg/ m L,检出限为 0 .4 μg/ m L。应用该法测定了人血清样品总蛋白含量。研究了实验条件的选择 。
In H 3BO 3-NaOH medium (pH 8.8),the complex of nickel(Ⅱ)-serum albumin yields a polarographic catalytic prewave at about -0.60V (vs.SCE). There is a linear relationship between the second order derivative peak height and the concentration of protein in a certain range. For BSA (bovine serum albumin),the linear range is 1～29μg/mL with the detection limit of 0.5μg/mL, and for HSA (human serum albumin),the linear range is 0.8～26μg/mL with the detection limit of 0.4μg/mL. The method has been used to the determination of total protein content in samples of human serum with satisfaction. The experimental conditions and the mechanism of the method were investigated.
出处
《分析仪器》
CAS
2004年第2期27-30,共4页
Analytical Instrumentation
基金
湖北省自然科学基金 ( 99J0 63 )
中南民族大学自然科学基金 ( YZZ0 3 0 0 3)
