摘要
研究了多孔壳聚糖球珠的制备条件,壳聚糖球珠的活化以及酶在球珠上的固定化条件;对固定化酶和游离酶的活性等进行了比较,发现壳聚糖球珠固定化脲酶的储藏稳定性优于游离酶,但催化活性小于游离酶,其米氏常数小于游离酶。应用所制备的固定化酶于流动注射分析废水中的尿素,线性范围为1.0×10^(-5)～5.0×10^(-3)g/mL,平均回收率(100±3.2)％,检出限1.0×10^(-5)g/mL。
The porous chitosan microspheres were prepared. Their activation and immobilization with urease were investigated. It is found that the immobilized urease exihibited storage stability better than the free urease, but with lower activity. The chitosan-immobilized uease was applied as a bioreactor in the determination of urea in waste water by flow injection analysis. The linear range of urea concentration was 1.0×10^(-5)～5.0×10^(-3) g/mL with the mean recovery being (100.5±3.2)% , detection limit was 1.0×10^(-5)g/mL
出处
《应用化学》
CAS
CSCD
北大核心
2004年第4期357-360,共4页
Chinese Journal of Applied Chemistry
基金
陕西省自然科学基金(01JK073)
关键词
脲酶
壳聚糖
固定化
流动注射
尿素测定
urease
chitosan
immobilization
bioreactor
urea determination
