摘要
目的 :探索酒精性肝病早期无创伤、灵敏和高效的检测方法。方法 :复制大鼠ALD模型 ,采用放免法和肝组织Mallory染色法动态检测大鼠血清中透明质酸 (HA)、层粘连蛋白 (LN)、Ⅳ型胶原 (CIV)的含量和肝组织内胶原的表达。结果 :ALD初期 ,血清中LN、CIV 和HA已呈现较高浓度 ,其中 ,HA的浓度随病程持续升高 (P <0 0 5 ,P <0 0 1) ,LN浓度在ALD初期变化不明显 (P >0 0 5 ) ,其后随造模时间延长而呈缓慢增长的趋势 ;CIV 在ALD初期呈快速增高的趋势 (P <0 0 5 ,P <0 0 1) ,随造模时间的延长而呈缓慢增加的趋势。病理检测 ,ALD发生一般经历酒精性脂肪肝和肝纤维化两个过程 ,酒精性肝纤维化主要病理特征是Disse间隙内胶原过度沉积 ,肝细胞内脂肪样变始终伴随ALD全过程。结论 :Ⅳ型胶原、LN和HA含量可作为ALD早期诊断的血清学指标 ,其中HA较为敏感。
Objective : To investigate the non-hurt, sensitive, and high-effective examination method of alcohol liver disease (ALD) Methods : The model of rat ALD was produced, the radio-immune analysis and liver tissue collagen stain were used to examine the changes continually Results : First, at the initial stages of ALD, the high level of LN, HA and C IV was observed( P <0 05, P <0 01) Among them, HA level raised with the developing of ALD rapidly and continually Second, during the whole process of ALD taking place, the quantity of collagen in hepatic Disse interspaces increased continually and some fatty droplets existed in the hepatic cell all the time Conclusion : The level of LN, HA and C IV may be the blood serum index in ALD early diagnosis, and perhaps HA is the best sensitive index among them
出处
《中国药物依赖性杂志》
CAS
CSCD
2004年第1期18-21,共4页
Chinese Journal of Drug Dependence
基金
国家自然科学基金重点资助项目 ( 30 130 2 2 0 )
