摘要
目的 测定鱼油软胶囊中二十碳五烯酸乙酯(EPA-E)和二十二碳六烯酸乙酯(DHA-E)的含量。方法 采用530 μm大口径毛细管柱气相色谱法,外标法测定EPA-E和DHA-E的含量。结果 EPA-E和DHA-E的保留时间分别为8.0 min和12.0 min,其标准曲线分别在0.52～1.22μg和0.58-1.36μg之间有良好的线性关系,回收率分别为97.1％和98.2％。结论 该方法专属性强,适用于鱼油软胶囊中EPA-E和DHA-E的定量分析。
OBJECTIVE To establish a 530 fan capillary gas chromatography method for the determination of eicosapentaenoic acid ethyl ester (EPA - E)and docosahexaenoic acid ethyl ester(DHA - E) in fish oil capsules.METHODS Samples were determined by 530 μm capillary gas chromatography with external standardization.RESULTS The retention time of EPA - E and DHA - E were 8.0 min and 12.0 min, respectively. The standard curves of EPA - E and DHA - E were linear in the range 0.52 to 1.22 μg and 0.58 to 1.36 μg, respectively. The average recoveries were 97.1 % for EPA - E and 98.2 % for DHA - E. CONCLUSION The method was sensitive and specific. It was suitable for analysis of EPA - E and DHA - E in fish oil capsules.
出处
《华西药学杂志》
CAS
CSCD
2004年第2期117-119,共3页
West China Journal of Pharmaceutical Sciences
