摘要
目的用气相色谱法测定鱼油多烯乙酯中二十碳五烯酸乙酯 (EPA E)和二十二碳六烯酸乙酯 (DHA E)的含量。方法采用气相色谱法 ,聚二乙二醇酯为固定相 ,涂布浓度为 10 % ,载体为ChromosorbWAWDMCS 80～ 10 0目的气相柱。结果EPA E平均回收率为 10 0 .31% ,RSD为 2 .5 4 % ;DHA E平均回收率为 10 3.37% ,RSD为 2 .76 %。结论此法操作简便、准确、重现性、稳定性好 。
PurposeThe aim is to determine the eicosapentaenoic acid ethyl ester and docosahexaenoic acid ethyl ester in fish oil ethyl polyenoic acid by gas chromatography (GC).MethodsGas chromatography, stationary phase: Chrosorb W AW DMCS 80～100 mesh coated with 10% DEGS.ResultsThe EPA E recovery was 100.31% and the RSD was 2.54%. The DHA E recovery was 103.37% and the RSD was 2.76%.ConclusionThis method was convenient,accurate reproducible and stable,so it was suitable for assay of eicosapentaenoic acid ethyl ester and docosahexaenoic acid ethyl ester in fish oil ethyl polyenoic acid.
出处
《中国生化药物杂志》
CAS
CSCD
2002年第2期72-74,共3页
Chinese Journal of Biochemical Pharmaceutics
