摘要
了解传染性非典型肺炎(severe acute respiratory syndrome,SARS)康复者体内冠状病毒特异性抗体IgG的产生水平及其与肺功能的关系。 方法:抽取305例SARS康复者康复后时期的血液,经病毒灭活后分离血清,用酶联免疫吸附法对冠状病毒特异性抗体IgG进行检测。另选取了180例健康者作为对照。 结果:81.3％(248/305)的康复者IgG抗体为阳性(抗体OD值为1.126±0.337),最长检测到康复165d的患者,该类抗体仍然呈阳性;18.7％(57/305)的康复者IgG抗体为阴性(抗体OD值为0.020 7±0.0264)。180例对照组中该类抗体全部为阴性(抗体OD值为0.019±0.010)。SARS康复者抗体阴性组与健康献血人群组之间不存在显著性差异(t=0.548,P=0.983);而上述两组与SARS康复者抗体阳性组之间存在显著性差异(t=12.321,P=0.000)。 结论:大多数SAPS康复者体内产生了冠状病毒特异的IgG抗体,并且这类抗体可能持续较长时间。
AIM: To study the specific antibody level of IgG against coronavirus and its relationship with lung function in convalescent patients with severe acute respiratory syndrome (SARS).
METHODS: The blood of 305 convalescent SARS patients was obtained, the blood serums were separated after virus inactivation, then the IgG antibody of coronavims was detected with enzyme-linked inununoadsordent assay (ELISA), another 180 healthy people were included as the control group. RESULTS: The positive rate of specific IgG antibody was 81. 3% (248/ 305) in convalescent SARS patients [optical density(OD) value was 1. 126 ±0. 337], this kind of antibody was still positive until the 165 d. The negative rate of IgG antibody was 18. 7% (57/305) in convalescent patients (OD value was 0. 020 7 ±0.026 4). The IgG antibodies were all negative in the 180 healthy people(OD value was 0. 019 ±0. 010) . There were no significant differences between the negative antibody group in convalescent patients with SARS and the normal control group (t =0. 548, P = 0.983), while the differences were significant between the above two groups and the positive antibody group( t = 12. 321, P = 0. 000).
CONCLUSION: Specific IgG antibody can be found in most of the convalescent patients with SARS, which may last for a long time.
出处
《中国临床康复》
CSCD
2004年第9期1702-1703,共2页
Chinese Journal of Clinical Rehabilitation
基金
国家863基金(2003AA208107)~~
