摘要
目的 :探讨非霍奇金氏淋巴瘤 (NHL)病理特点、免疫表型及与慢性淋巴腺炎和增生性淋巴腺炎 (RH)免疫表型的不同表达。方法 :对 2 0 6例淋巴结肿大患者进行淋巴结穿刺 ,取穿刺液进行染色镜检 ,作出初步诊断。而后采用流式细胞仪(FCM)检测各患者淋巴结穿刺液中淋巴细胞免疫表型。结果 :①经形态学检查慢性淋巴腺炎患者 95例 ;增生性淋巴腺炎患者4 0例 ;NHL患者 71例。② 71例NHL中B NHL 5 3例 ,占 74 6 4 % ;T NHL 12例 ,占 16 90 % ;CD3阳性 15例 ,占 2 1 13% ;CD4阳性6例 ,占 8 4 5 % ;CD34阳性 30例 ,占 4 2 2 5 %。③NHL各抗原表达结果与增生性淋巴腺炎、慢性淋巴腺炎各抗原表达结果均有显著差异 (P <0 0 5 )。结论 :免疫表型检查有助于确定绝大多数NHL病例、肿瘤增生细胞的T或B性质及克隆性 ,免疫表型检查可作为NHL鉴别诊断的辅助指标。
Objective:To investigate the relationship between clinicopathologic features and immunophenotypes in non-Hodgkin's lymphoma (NHL), and to the different expression of immunophenotypes in NH、RH、and chronic lymphadenitis.Methods:Puncture lymphatic from 206 cases and diagenose lymph through microscope, then immunophenotypes in 206 patients were detected quantitatively with FCM.Results:①95 cases were chronic lymphadenitis, 40 cases were RH, 71 cases were NHL.②The B-NHL were 53 cases(74.64%);T-NHL were 12 case (16.90%) in 71 cases of NHL.The straining rates of poly clonal CD3、CD4、CD34 were 21.13%、8.45%、42.25%.③There were significant difference in immunophenotypes among NHL、RH and chronic lymphadenitis( P <0.05).Conclusion:The results showed that analysis of the immunophenotype of NHL is helpful to ensure the character of T cell or B cell.It is helpful to differentiate the NHL too.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2004年第3期209-210,共2页
Chinese Journal of Immunology
