摘要
石刁柏(Aspagus officinalis L.)体细胞胚状体是由单个具极性分化的胚性细胞分裂、发育而来。经透射电镜观察,早期胚性细胞与分生细胞的形态相同,形小、核大、质浓、多个小液泡、细胞器丰富、壁薄。胚性细胞进一步成熟,出现极性,细胞核偏居一端,细胞质、细胞器多聚于核端,另一端被大液泡充满。胚性细胞的极性出现与受精卵相似。组织切片表明:胚性细胞经2细胞、4细胞、8细胞、具胚柄或不具胚柄的多细胞原胚、球形胚、梨形胚、长形胚、子叶分化期胚(一片子叶)到成熟胚。同时,观察到类似于双子叶植物胚胎发育过程中的心形胚和鱼雷形胚。此外尚能观察到连体胚、多胚、次生胚等畸形胚。
The single cell was the first cell of somatic embryoid in Asparagus officinalis L.. Electron microscopic observations revealed that early embryogenic ceils and meristematic cells had the same characteristics in cell shape and structure. Th'e embryogenic cell was sn,all in size, with large nucleus, dense cytoplasm, thin wall, lots of small vacuoles, and rich in organelles. At later stage, the polarity of embryogenic cell appeared, i.e. nucleus situated at one end of the cell, while the other end was occupied by a large vacuole. The polar type of the embryogenic cell was similar to that of a zygote. Light microscopic observations revealed that the embryoids were sequentially differentiated through 2-cells, 4-cells, s-cells, multicellular proembryo, globular- shaped, pear-shaped, rod-shaped, cotyledonary-differentiated and mature embryo stages. The heart-shaped and torpedo-shaped stages were observed during the early stage of embryogenesis. In addition, a typical embryoids were also found in vitro.
基金
国家自然科学基金资助课题
关键词
石刁柏
细胞组织学
体细胞胚发生
Asparagus officinalis L
S matic embryogenesis
Embryogenic cell
Cyto histology
