摘要
以临泽小枣子叶切块为外植体 ,在附加 0 .2 mg/L IBA+ 1 .0 mg/L 6- BA的 MS培养基上 1周后切块边缘可诱导出白色胚性愈伤组织 ,继续培养 1个月后愈伤组织中产生体细胞胚。体细胞胚发生不同步 ,经历球形胚、心形胚、子叶胚等阶段 ,与合子胚发育途径相似。组织切片表明胚性愈伤组织细胞体积小 ,细胞核大 ,细胞质浓 ,细胞排列紧密 ;而非胚性愈伤组织细胞体积大 ,细胞核小 ,细胞质稀薄。子叶胚时期体细胞胚内部出现维管束 ,并观察到螺纹导管。
The somatic embryos were induced from embryonic calli that were induced from cotyledon explants of rare breed of jujuba Linze Chinese date.The embryoids successively differentiated through globular shaped,heart shaped and cotyledon stages,which was similar to the development of zygotic embryo.The histological study revealed that the cells of non embryonic calli derived from leaf were large in size,with small nucleus,thin cytoplasm,however,the cells of embryonic were small in size,with large nucleus and dense cytoplasm.Vascular system and vessel element in cotyledonary embryoid was also observed.
出处
《西北植物学报》
CAS
CSCD
2001年第1期142-145,T006,共5页
Acta Botanica Boreali-Occidentalia Sinica
关键词
枣树
组织培养
胚性愈伤组织
体细胞胚发生
组织学特征
Zizyphus jujuba Mill.
tissue culture
embryonic callus
somatic embryogenesis
histological characteristics
