摘要
以红缨海棠当年生新枝为起始外植体建立了离体再生体系,并进行了不定芽及不定根分化的细胞组织学研究。结果表明:在添加6-BA1.0 mg/L和NAA0.05 mg/L的N6培养基中培养30天,丛生芽分化效果最佳,其分化率达100%。在添加6-BA0.5 mg/L和NAA0.05 mg/L的N6培养基中,丛生芽增殖效果最好。伸长的不定芽接种在附加NAA和IBA共0.5 mg/L(两者质量比为1∶1)的1/2N6生根培养基中,先经6天暗培养,再经过24天培养后生根率可达100%,平均不定根数量达8条/株,平均不定根长0.3 cm,最长达1.0 cm。生根苗移栽至混有珍珠岩、蛭石和草炭土配比为1∶1∶1(V∶V∶V)的基质中,60天后成活率约为80.0%。而细胞组织学研究表明,带顶芽茎段在NAA1.0 mg/L和6-BA0.05 mg/L共同作用下,不定芽起源形式既有内起源,又有外起源。而不定根起源于茎维管形成层细胞,属内起源。
The culturing stems with newborn buds were used as explants to establish in vitro culture and plant regeneration system of Malus spectabilis ＇Hongying＇ and the cytohistology of the adventitious bud and adventitious root were studied.The results showed that the differentiation rate of multiple shoots was reached to 100.0% when the explants were cultured in a N6 medium contained 6-BA 1.0 mg/L and NAA 0.05 mg/L for 30d.And the proliferation was the best when the multiple shoots were subcultured in a 1/2 N6 medium with a 1∶1（m∶m）mixture of 6-BA 0.025 mg/L and NAA 0.025 mg/L.the rooting ratio were reached to 100.0% when the adventitious buds were induced in the 1/2 N6 medium with 0.5 mg/L NAA＋IBA（1∶1）（m∶m）for 30 days（6-day dark pre-cultivation）.Each stem had eight roots with a length of 0.3 cm in average,and the longest root was 1.0 cm.The plantlet could be transplanted to a 1∶1∶1（V∶V∶V） mixture of perlite,vermiculite and peat soil and the survival rate of the regeneration plants were over 80.0%.Cytohistologic examination showed that the addition of NAA 2.0 mg/L and 6-BA 0.10 mg/L stimulated differentiation of the stems with apical buds.The adventitious buds were developed from either the superficial meristemoids or the meristememyic regions deep within pith,while the adventitious roots emerged from the cambium of stem were endogenesis,belonging to the meristememyic region deep within pith.
出处
《林业科技开发》
2010年第6期23-28,共6页
China Forestry Science and Technology
基金
江苏省科技支撑项目(编号:BE2008394)
关键词
红缨海棠
组织培养
茎段
细胞组织学
Malus spectabilis ＇Hongying＇
tissue culture
stem-segment
cytohistology
