摘要
设立不同的 PPT浓度梯度分别对来自籼稻珍汕 97B和粳稻台北 30 9、日本晴成熟种子胚的愈伤组织进行天然抗性试验 ,并对转 bar基因的幼苗进行了 Basta涂抹试验 ,以便对转化苗早期初步鉴定。结果表明 :籼稻和粳稻均表现出一定的对 PPT的天然抗性 ,转 bar基因水稻在筛选抗性愈伤时 ,以浓度为 1 0 mg/ L 的 PPT较为合适 ;而粳稻则必须提高至2 0 mg/ L为宜。叶片涂抹 Basta鉴定转化植株时 ,以 0 .1 2 5 %的浓度方才有效 ,且籼。
This experiment tested the natural herbicide resistance in calli, which initiated from mature seed scutella of Indica rice ZhenShan 97B and Japonica rice TaiBei 309 and Nipponbane, by using different densities of PPT. Transgenic rice seedling leaves was swearing over by basta to appraise herbicide resistance of the young plant. The results showed that both Indica rice and Japonica rice have some natural herbicide resistance. The density of 10mg.L -1 PPT is suitable to select resistant calli in bar transgenic rice. The concentration of 0.125% basta can initially appraise seedlings of transgenic rice.
出处
《种子》
CSCD
北大核心
2004年第3期7-10,共4页
Seed
基金
国家科技部资助项目 (J99- A- DD2 )
