摘要
本文报导了以上农香糯为受体植物,用吸涨法、组织培养法和花粉管通道导入等方法将小牛胸腺 DNA 导入上农香糯.所得结果表明:1.外源 DNA 能增加剑叶宽度;2.外源 DNA(模糊多基因)能使上农香糯对稻瘟病和白叶枯病的抗性提高.对照对稻瘟病为8级感病,白叶枯为中感,而导入模糊多基因后的上农香糯对稻瘟病的感病性已降至7级和6级,对白叶枯病已为中抗.
Plant gene enginecring not only brcaks down obstacles to scxual cross,overcomes diffi- culties in distant hybridization,but also combines the useful gene from any creatures or artificially modified genes as well to create new species.It is important for breeders to realize the value of gene engineering and utilize it,but classical gene engineering is capital consuming ,needing well-trained workers and modernized equipment,so scientists devoted to use gene engineering in attaining crop improvement goals put emphasis on studying exogenous DNA introduction techniques with species recipient. We try to introduce DNA from calf thymus to Shangnong aromatic glutinous rice by fol- lowing ways: 1.Soaking seeds in 100ppm,300ppm,500ppm exogenous DNA solution,respectively; 2.Tissue culture,medium being Ms+KT0.5+2.4-D_2+DNA100;Ms+KT0.5+2.4-D_2+ DNA300;Ms+KT0.5+2.4-D_2+DNA500,respectively; 3.Pollen tube way,infecting 0.1-0.2ml 300ppm DNA solution per flower; 4.Test grafting. Results show: 1.Exogenous DNA has significant effect on the width of flag leaf,but not on its length; 2.Exogenous DNA has no significant effect on the peroxidase activity of recipient plant; 3.Introducing exogenous DNA could heighten resistance of recipient plant on rice-blast and rice—stripe,especially on the latter; 4.There are no significant differents among the effects of three exogenous DNA cocentrations.
基金
本课题为上海市科委自然科学基金
关键词
多基因
上农香糯
抗病性
组织培养
Vague multigene
Disease resistance
Shangnong aromatic glutinous rice
