摘要
用Bar基因通过基因枪法轰击京引119未成熟胚，得到抗除草剂Basta的转基因植株，经加代和选择得到抗除草剂的稳定系TR4，其基本农艺性状与京引119相仿。用TR4与4个粳稻品种杂交进行遗传分析表明，TR4的抗除草剂特性由一对显性基因控制，符合孟德尔遗传规律。该外源基因可通过有性杂交转育，在杂交稻和常规直播稻的选育上有较大的利用价值。
Immature embryos of rice variety Jingyin ll9 were treated with bar gene via particle bombardment. Herbicide resistant transgenic rice plantlets were obtained. The stable herbicide resistant transgenic rice line TR4,which possesses agronomic characters similar to Jingyin 1l9,was consecutively selected from the self-pollinated progenies of above transformed plant. Genetic analysis of the crosses of TR4 with four japonica rice varieties demonstrated that the herbicide resistance of TR4 was controlled by a pair of dominant genes and accorded with Mendelian segregation. Successful transbreeding of bar gene by sexual hybridization is valuable in selection of hybrid rice and direct seeded rice.
出处
《中国水稻科学》
CAS
CSCD
北大核心
1999年第3期173-175,共3页
Chinese Journal of Rice Science
关键词
水稻
抗除草剂
转基因植株
遗传分析
rice
herbicide resistance
transgenic plant
genetic analysis
