摘要
目的 :测定川芎挥发油中藁本内酯的含量。方法 :采用RP HPLC法 ,内标法定量 ,内标物尼群地平 ,HypersilODS2 色谱柱 (4.6mm× 2 0 0mm ,5 μm) ,流动相为甲醇 乙腈 水 (33∶2 1∶4 6 ) ,检测波长 2 75nm。 结果 :藁本内酯在 2 .92～ 2 9.2mg·L-1线性关系良好 ,藁本内酯在川芎挥发油中的含量为 34.79% ,藁本内酯平均回收率 95 .1% ,RSD为 2 .3%。结论 :本方法简便 ,准确 ,重现性好 ,可用于藁本内酯的含量测定。
Objective: To determine Ligustilide in volatile oil from Ligustrcum chuanxiong with RP-HPLC. Method: ODS 2 column (4.6 mm×200 mm,5 μm)was used and nitrendipine was used as internal standard. The mobil phase consisted methanol,acetontrile and water(33∶21∶46). The ligustilide was at 275 nm. Result: The linear range was 2.92～29.2 mg·L -1 for ligustilide. The average recovery of ligustilide was 95.1% and RSD was 2.3%. Conclusion: This method is simple and can be used to determine ligustilide with satisfactory accuracy and reproducibility.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2004年第2期154-157,共4页
China Journal of Chinese Materia Medica
