摘要
报道了藁本中５种成分：阿魏酸（ｆｅｒｕｌｉｃａｃｉｄ）、莨菪亭（ｓｃｏｐｏｌｅｔｉｎ）、正丁苯酞（３ｂｕｔｙｌｐｈａｔｈａｌｉｄｅ）、藁本内酯（ｌｉｇｕｓｔｉｌｉｄｅ）、藁本内酯二聚体（ｄｉｌｉｇｕｓｔｉｌｉｄｅ）的反相高效液相色谱法测定。采用梯度洗脱法，用ＨｙｐｅｒｓｉｌＣ１８键合相柱，乙腈（含１２５％ＣＨＣｌ３）—水（以ＣＨＣｌ３饱和）为流动相，苄噻嗪为内标，２８４ｎｍ为检测波长，二极管阵列检测器检测峰纯度，方法快速、简便。
A RP HPLC method was established to separate and determine five constituents in plants of genus Ligusticum by using an ODS column (Hypersil C 18 , 5 μm) and acetonitrile (containing 1 25% CHCl 3) water (saturated with CHCl 3) as mobile phase for gradient elution. The five constituents were ferulic acid, scopoletin, 3 butyl phthalide, ligustilide, and diligustide. They were detected at 284 nm. Peak purity was monitored by photodiode array detector. Benzthiazide was used as the internal standard. The method is simple, fast, sensitive and reproducible.
出处
《药学学报》
CAS
CSCD
北大核心
1996年第8期622-625,共4页
Acta Pharmaceutica Sinica
关键词
藁本属
高效液相色谱法
阿魏酸
莨菪亭
化学成分
Ligusticum
HPLC
Ferulic acid
Scopoletin
3 Butyl phthalide
Ligustilide
Diligustilide
