摘要
目的 探讨有机磷 (OP)中毒家兔脑干损伤的病理机制。方法 :2 4只青紫蓝家兔分 4组 :敌百虫 (1 0LD ,1 1 1 2 0mg/Kg)组 ,久效磷 (1 0LD ,2 2 2 4mg/Kg)组 ,甲基对硫磷 (1 0LD ,74 1 0mg/kg)组和正常对照组 ,每组 6只。中毒 6、 2 4h各取脑干组织用于组织病理学观察及制备组织匀浆。用酶抑制法测脑干组织游离有机磷毒质 (FOP)含量 ,用放射免疫法测组织中肿瘤坏死因子 α (TNF -α)、白细胞介素 1 β (IL 1 β)及血栓素B2 (TXB2 )含量。结果 中毒后 6、 2 4h脑干组织出现严重的病理损伤 ,可见脑干组织水肿、出血 ,神经细胞变性坏死 ,形成鬼影细胞 ,并见神经纤维脱髓鞘改变。同时 3种OP在脑干组织中可检出不同浓度的FOP。中毒后 6h ,脑干TXB2 含量高于正常 (t =3 984 3,P <0 0 1 ) ,2 4h与 6h比较差异有显著性 (t =2 5 4 32 ,P <0 0 5 ) ;TNF α、IL 1 β在各时相与正常比较差异无显著性 (P >0 0 5 )。结论 重度有机磷中毒可致动物脑干组织出现严重的病理损伤 ,除中毒酶机制以外 ,FOP和TXA2
Objective To explore the molecular and pathological mechanism of injury of brain stem injury in rabbits poisoned with organophosphates.Methods 24 livid blue rabbits were divided into 4 groups:trichlorfon(10LD,1 112 0 mg/kg),monocrotophos (10LD,22 24 mg/kg)and methy1 parathion(10LD,74 10 mg/kg)groups and control group.Every group consists of 6 rabbits.The organophosphates were administered by subcutaneous route.The specimens of brain stem were collected for pathological examination and preparation of tissue homogenate at 6 and 24 h after poisoning.The levels of free organophosphorus poisons(FOP) in brain stem were determined with enzyme inhibition method.The concentrations of tumor necrosis factor alpha(TNF α),interleukin 1 beta(IL 1β),Thromboxane B 2(TXB 2) in the brain stem were measured by radio immunoassay technique.Results Severe pathological changes of brain stem were observed at 6 and 24 h after poisoning,such as tissue edema,and hemorrhage,with degeneration and necrosis cells and neurofibrillar demyelination.There were different concentrations of FOP of trichlorfon,monocrotophos and methy1 parathion in the brain stem.TXB 2 level in the brain stem at 6 h after poisoning was significantly higher than that in control group ( t = 3 9843, P <0 01),and a significant difference was also found between 6 and 24 h after poisoning( t =2 5432, P <0 05).TNF α and IL 1β concentrations were not higher than those in control group( P >0 05).Conclusion Severe organophosphorus poisoning can result in severe pathological injury of the brain stem in animals.FOP and TXA 2 play important roles in the injury of the brain stem.
出处
《中华急诊医学杂志》
CAS
CSCD
2003年第6期391-393,I001,共4页
Chinese Journal of Emergency Medicine
基金
山东省卫生厅医疗攻关项目 ( 1998CAICFA2 )
