摘要
目的 探讨不同有机磷杀虫剂 (OP)在中毒家兔血浆、胆汁分布的过程及意义。方法 72只青紫蓝家兔分为 3组。敌百虫 (5 5 6 0mg/kg)、久效磷 (11 12mg/kg)、甲基对硫磷 (3 7 0 5mg/kg)。每组 2 4只。家兔中毒经皮下给药。中毒后 1、6、2 4、96h取血和胆汁 ,分离血细胞和血浆。用DTNB酶动力学法测乙酰胆碱酯酶 (AChE)活力 ,酶抑制法测游离有机磷毒质 (FOP)含量。结果 中毒后 1～ 96h ,血浆中FOP浓度与时间呈负相关 (敌百虫、久效磷、甲基对硫磷 ,r分别为 - 0 74,- 0 5 5 ,- 0 69,P值均 <0 0 1)。胆汁中敌百虫、久效磷FOP浓度与时间呈负相关 (敌百虫、久效磷r分别为 - 0 97,- 0 71,P值均 <0 0 1) ;甲基对硫磷无直线相关 (r=- 0 14,P >0 0 5 )。中毒后 96h ,血浆中无FOP残留时 ,胆汁中仍有高浓度的FOP ,敌百虫、久效磷、甲基对硫磷分别为 (3 0 0 3± 174 44、3 62 8± 13 6 62、10 1 0± 75 85 )IU/L。结论 胆囊中的胆汁是OP中毒动物FOP最重要的贮存库 ,FOP在胆汁中可存留96h以上 ;FOP在血。
Objective To study the process and significance of the distribution of free organophosphorus poisons (FOPs) in the blood and bile of rabbits poisoned with organophosphates Methods Seventy two livid blue rabbits, male, 2-2 5 kg in weight, were divided into 3 groups: trichlorfon (556 0 mg/kg), monocrotophos (11 12 mg/kg) and methyl parathion (37 05 mg/kg) Each group consisted of 24 rabbits All organophosphates were administered by subcutaneous route. Blood and bile were collected at time intervals of 1, 6, 24, 96 hours after administration. Blood cells and plasma were separated Acetylcholinesterase (AChE) activity was measured with dithiobisnitrobenzoic acid (DTNB) enzyme kinetic method The levels of FOPs in plasma and bile were determined with enzyme inhibited method Results From 1 h to 96 h after administration negative correlation was found between time and FOP concentration in plasma (trichlorfon, r =-0 74, P <0 01; monocrotophos, r = -0 55, P <0 01; methyl parathion, r =-0 69, P <0 01), and it was also found in bile between time and FOP concentration of trichlorfon( r =-0 97, P <0 01) and monocrotophos ( r =-0 71, P <0 01) There is no linear correlation between time and concentration of methyl parathion in bile( r =-0 14, P >0 05). When FOPs in plasma were not detectable at 96 h after administration, high levels of FOPs could still be detected in bile [trichlorfon (300 3±174 44) IU/L; monocrotophos (362 8±136 62) IU/L; methyl parathion (101 0±75 85) IU/L] Conclusion The bile is the most important store for FOPs in animal FOPs can exist in bile over 96 h The process of poisoning is a changing process of absorption, distribution and redistribution and it is different owing to various physical and chemical properties of organophosphates
出处
《中华内科杂志》
CAS
CSCD
北大核心
2002年第12期795-797,共3页
Chinese Journal of Internal Medicine
基金
山东省卫生厅医疗攻关资助项目(1998CAICFA2 )
