摘要
目的 :以氧化前胡素、欧前胡素、总香豆素含量为试验指标 ,优化白芷中香豆素类成分的超临界CO2 萃取工艺。方法 :通过正交试验 ,采用高效液相色谱法测定氧化前胡素、欧前胡素的含量 ,采用极差方差和逐步非线性回归对试验数据进行分析。结果 :白芷中香豆素类成分的超临界CO2 的最佳萃取工艺为萃取温度 5 0℃、萃取压力 2 1MPa、萃取时间 3.5h、分离压力 6 .0MPa、粉碎度 2 0目 ;实验值和模型计算值的相对误差小于 5 %。结论 :萃取压力、温度、时间、药材的粉碎度等工艺参数对试验指标有显著性影响 ,而且部分因素之间存在交互作用 ,回归模型具有较高的预测精度。
AIM: To optimize the extraction process for coumarins in Angelica Dahurica by supercritical CO 2 as guided by the contents of oxyimperatorin, imperatorin and the total coumarins in the extract. METHODS: HPLC was applied to determine the contents of oxyimperatorin and imperatorin. orthogonal design was applied to optimize SFE process. Range and variance analysis as well as stepwise nonlinear regression analysis were applied to deal with experimental results. RESULTS: The optimum process was established as following: 21MPa as extracting pressure, 50℃ as extracting temperature, 3.5h as extracting time, 20 mesh as pulverized degree and 6.5MPa as separating pressure. The relative error between experimental data and calculated value was less than 5%. CONCLUSION: Changes in extracting pressure, temperature, time, pulverized degree would affect the extracting results remarkably. Some factors had interactions on the extraction of coumarins in Angelica Dahurica . Regression equation was reasonable and could forecast results precisely.
出处
《中成药》
CAS
CSCD
北大核心
2004年第2期90-93,共4页
Chinese Traditional Patent Medicine
关键词
超临界萃取工艺
白芷
香豆素
正交设计
逐步回归
supercritical extraction process
Angelica Dahurica
coumarins
orthogonal design
stepwise regression analysis
