摘要
以大肠杆菌JM10 5(E .ColiJM10 5)为模拟体系 ,系统研究一种新型聚阳离子型表面接触抗菌材料及其单体的抗菌过程和机理 .采用平板计数法测定该单体的最低抑菌浓度以衡量其抗菌能力 ,采用扫描电镜法和 β 半乳糖苷酶活性考察该单体对大肠杆菌的抑制作用过程及其机理 .分别采用静态和动态吸附法考察聚阳离子型表面接触抗菌材料对大肠杆菌的吸附性能 ,测定吸附在材料表面菌体TTC 脱氢酶的活性和菌体呼吸活性以揭示该材料的抗菌作用机理 ,并采用电镜观察抗菌纤维表面菌体形态随时间的变化 .结果表明 ,聚阳离子型表面接触抗菌材料的单体可导致大肠杆菌细胞壁破裂 ,由此破坏细胞膜并导致细胞凝聚而死亡 .该消毒剂的灭菌过程由吸附和杀灭两步构成 ,前者为快速过程 。
Surface contact disinfecting technique is a newly developed method for water sterilization.An experimental protocol to elucidate the anti bacterial p r ocess and mechanism was proposed and applied to a novel surface contact disinfec tant made of cellulose fiber grafted with DMAE BC,using E.Coli,as a model b ac terium.The minimum inhibition concentration (MIC) of the monomer,defined as a concentration at which the growth of E.Coli.in water is completely inhibite d ,was determined by the plate counting method.MIC was then used as an index to ch aracterize disinfection capability of the monomers.The disinfection mechanism o f the monomer was further investigated by measuring the activity of β gala ctosid ase and observing the cell morphology with a scanning electron microscope (SEM). To elucidate the disinfection process and mechanism of the grafted fiber,the s teady state and dynamic adsorption of E.Coli.on the fiber was examined,resp e ctively.The TTC dehydrogenase activity and respiration activity of E.Coli.a dsor bed on the fiber surface were also determined.The results showed that the steri lization process by the grafted fiber was composed of two steps,in which the ads orp tion of E.Coli.bacteria on the fiber surface driven by electrostatic interac tion was a fast process and cell lysis was a relatively slow process.
出处
《高分子学报》
SCIE
CAS
CSCD
北大核心
2004年第1期107-113,共7页
Acta Polymerica Sinica
