摘要
首次发现低等动物地龙(Eisenia foetida,亦称蝗蚓)体内含有血小板活化因子(PAF),含量随季节变化,平均达10.7±6.1pmol/g湿重(或1.1±0.5μmol/mol磷脂)。磷脂成分中,PAF前体物质1-O-烷基-2-酰基(长链)-sn-甘油-3-磷酸胆碱占磷脂酰胆碱的比例高达61.4％。并证实地龙体内含有与哺乳类动物相同的两种PAF合成酶蛋白。当针刺、切断等伤害性刺激时,PAF水平显著升高。此结果提示PAF是一种初始的活性介质,在地龙这类低等动物体内也参与机体的病理、生理应急反应。本研究为阐明中药地龙的降血压等药理作用机理提供新线索。
Platelet-activating factor (PAF) was for the first time confirmed to exist in a lower animal, earthworm (Eisenia foetida). It amounts to 10. 7±6. 1 pmol/g wet body weight, andvaried seasonally. Phospholipid analysis revealed that 1-O-alkyl-2-acyl-sn-glycerophosphocholine, astored form of PAF precursor, accounted for 61.4% of the choline glycerophospholipids. Two kindsof enzyme activities operating in PAF generation in mammalian cells were also detected from this speci-es. The PAF level increased markedly under some injurious stimuli such as cutting,and pricking. Theresults suggest that PAF may be a primary mediator involved in pathological and physiological reac-tions even in lower animal like earthworm. The findings also cast a new light on the mechanisms under-lying the antihypertensive and other effects of the Chinese medicinal earthworm, pheretima.
出处
《药学学报》
CAS
CSCD
北大核心
1992年第12期886-890,共5页
Acta Pharmaceutica Sinica
关键词
血小板
活化因子
蚯蚓
生物合成
Platelet--activating factor
Earchworm
Biosynthesis
Phospholipid analysis
