摘要
用高效液相色谱法测定了地龙中的次黄嘌呤和黄嘌呤的含量.色谱柱:μ-BOndaPak C_18(10μm,3.9mm×00mml); 流动相:甲醇 -0.0lmol/L磷酸二氢铵(7:93);流速:1.2ml/mins;检测波长:254nm.方法重现住和国收率较好,为地龙的质量研究提供了有效的方法.
A HPLC method for the determination of hypoxanthine and xan-thine in Lumbricus was established. The separation was performed on a μ-Bonda-pak C18 (10μm, 3.9mm×300mm) colamn with a mobile phase of methanol-0.01mol/L NH4H2PO4 ( 7 : 93) . The flow rate was 1.2ml/min and the detection was at 254nm. The method displayed good producility and high recovery, and it provides an effective way for the study of Lumbricus.
出处
《华西药学杂志》
CAS
CSCD
北大核心
1994年第3期179-181,共3页
West China Journal of Pharmaceutical Sciences
关键词
地龙
次黄嘌呤
HPLC
Lumbricus Hypoxanthine Xanthine HPLC
