摘要
目的 :探讨补阳还五汤一次和连续灌服给药对大鼠实验性动脉血栓形成前后动脉血中血小板活化因子 (PAF)含量的影响。方法 :将雄性Wistar大鼠分别随机分为 3组。用药组一次或连续 (1 4天 ,每天 1次 )灌服补阳还五汤水煎液 (3 2 g/kg、1 6g/kg) ;对照组一次或连续 (1 4天 ,每日 1次 )灌服地塞米松片溶液 (1mg/kg、0 5mg/kg) ;空白组均灌服等体积蒸馏水(1 0ml/kg)。末次给药 2h后 ,复制实验性动脉血栓模型 ,用PAF含量的生物测定法 ,测定血栓形成前后动脉血中PAF含量和血栓OT值、血栓干重及血栓 -体重指数。结果 :补阳还五汤一次给药对血栓形成前后动脉血中PAF含量和血栓OT值无明显影响 ;而连续给药能明显降低血栓形成后动脉血中PAF含量 (P <0 0 5 ) ,并明显提高动脉血栓OT值 (P <0 0 5 ) ,降低血栓干重和血栓 -体重指数 (P <0 0 5 )。结论 :补阳还五汤对病理状态下PAF代谢有一定的拮抗效应 ,并对动脉血栓的发生、发展有一定的抑制作用。
Objective: To explore the effect of Buyang Huanwu Decoction (BHD) on arterial blood platelet factor (PAF) content in pre and post arterial thrombosis of rats. Methods: Male Wistar rats were randomized into 3 groups, the BHD group treated with BHD 32 g/kg, or with BHD 16 g/kg daily for 14 days consecutively, the control group treated with dexamethasone 0.5-1 mg/kg and the blank group treated with distilled water 10 ml/kg. Arterial thrombosis was duplicated in the animals 2 hours after final medication, the blood content of PAF (by bioassay), dry weight (W) and occlusion time (OT) of thrombus, and weight of thrombus/body weight (TW/BW) ratio were observed. Results: Consecutive BHD treatment could markedly lower the arterial blood content of PAF after thrombosis, increase the OT value of thrombus, reduce the dry weight of thrombus and the TW/BW ratio (P<0 05), while no influence was found in rats treated with BHD once. Conclusions: BHD has certain antagonistic effect on pathologic PAF metabolism, and inhibitory effect on pathogenesis and development of arterial thrombosis.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1998年第12期730-732,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
广东省中医药管理局科研基金资助课题!(No.930 39)
国家中医药管理局科研基金资助课题!(No .93C0 2 9)
关键词
补阳还五汤
实验性血栓
血小板活化因子
中药
Buyang Huanwu Decoction
experimental thrombosis
platelet activating factor
