摘要
取14只健康SD大鼠,分别以2 ％双苯甲亚胺(Bb)或3％碘化丙啶(PI)注入近胃小弯处胃体前壁或幽门部前壁的浆膜下,逆行追踪胃前壁的感觉神经的起源。结果表明:在双侧迷走神经结状节和双侧Th_1～L_1脊神经节观察到呈弥散分布的Bb标记细胞,双侧结状节中,左侧Bb标记细胞数量明显多于右侧;在双侧Th_1～L_1脊神经节中,Bb标记细胞集中出现于Th_5～Th_(10)脊神经节,双侧脊神经节出现Bb标记细胞数目相近。上述部位未见PI标记细胞,推侧PI标记特性不仅与荧光标记物运送距离有关,而且可能与注射部位的组织结构有关。
Separate 2% Bb and 3% PI aqueous solutions were injected beneath serosa of tne anterior wall of the gastric corpus or the pylorus antrum near the lesser of curvature in rats to the trace the origin the afferent nerve of the region. The results showed that Bb-labeled cells were found in both nodose ganglia (more cells on the left than on the right side) and in the Tl-Ll spinal ganglia (mainly in T5-T10) . These labeled cells can be referred to as the sensory neurons innervating the concerned parts of the anterior gastsic wall. However, no Pi-labeled cells were found in the above mentioned ganglia, which may perhaps be attributed to the limited distance the fluorescent marker can go and to the peculiar tissue structure of the injected sites.
出处
《徐州医学院学报》
CAS
1992年第4期266-270,共5页
Acta Academiae Medicinae Xuzhou
关键词
荧光标记法
神经支配
胃
retrograde fluoresceoce tracing
sensory innervation
gastric corpus
pylorus antrum
nodose ganglion, spinal ganglion
