摘要
在大白鼠侧脑室(LV)注射碘化丙啶(PI)后,动物均表现明显异常,有躯体震颤、共济失调及眼球震颤等。脑组织内 PI 荧光的强度,是以注射部位及脑室为中心向脑表面的方向逐渐减弱,在前脑可达同侧尾、壳核的最外侧部。并选择性地标记隔区的隔海马核、中脑多巴胺(DA)细胞 A_(8-9-10)群、中缝背核、中缝正中核、中脑尾份内侧丘系内及其背侧的神经细胞,以及小脑的 Purkinje 细胞,均为双侧标记。其他部位则未见明显标记。结合 Faglu 荧光组织化学显示,几乎全部中脑 DA 细胞均显 PI 荧光。A_(8-9-10)内未见非 DA 细胞标记。在 LV 注射 PI 前切断一侧前脑内侧束,可见 PI 堆积于近切断处远端的儿茶酚胺纤维内,而近端无堆积。LV 注射伊凡思蓝(EB)、溴乙啶或 DAPI,动物均未出现明显异常。EB 和 DAPI 在脑内远隔部位虽也可见神经元标记,但其分布与 PI 的标记明显不同。上述结果除进一步证实了 LV 注射 PI,可引起动物明显的异常表现及选择性被 Purkinje 细胞摄取外,还表明,1.PI 能从脑脊液内进入脑实质,并在脑内广泛扩散;2.选择性地标记脑内一些特定部位的神经细胞,其中在 A_(8-9-10)内的标记细胞均系 DA神经元;3.这些 DA 细胞是通过轴突摄取 PI 及逆行轴浆运输而标记的。 ‘
Injections of propidium iedide (PI) into the lateral cerebral ventricle (LV) ofthe rat resulted in a prominent abnormality characterized by tremor,ataxia,andnystagmus.The intensity of PI fluorescence in the parenchyma of the brain fadedgradually away from the injection site and ventricles to the surfaces of the brain. In the forebrain it was seen that PI fluorescence reached the most lateral part ofthe ipsilateral caudate putamen nucleus.A constant neuronal labeling was observedin the septohippocampal nuclei,the A8-9-10 dopaminergic cell groups of themidbrain,the dorsal raphe nucleus,the median raphe nucleus,neurons within anddorsal to the medial lemniscus of the caudal midbrain,and Furkinje cells of thecerebellum.This neuronal labeling was bilateral.No distinct labeling was seen inother areas of the brain.Combined with Faglu histofluorescence,it was found thatalmost all of the dopaminergic neurons in the midbrain exhibited PI fluorescence.No labeled non-dopaminergic neuron was seen in A8-9-10.With a transection ofthe unilateral medial forebrain bundle,a prominent accumulation of PI fluorescencewas seen within the distal segments of catecholaminergic fibers near the transection,but no accumulation of PI was seen in the proximal segments.With LV injectionof Evans blue(EB)or DAPI or ethidium bromide,animals did not exhibit anyvisible abnormality.In animals with LV injection of EB or DAPI,although somelabeled cells were seen in the distant areas of the brain,their distribution wasdistinctly different from that of PI labeling.The above results indicate that besidesconfirming the LV injection of PI results in a prominent abnormality and PI isselectively uptaken by Purkinje cells,we have found that:a)PI is able to enter theparenchyma from the cerebrospinal fluid and diffuse widely in the brain;b)LVinjection of PI results in a selective labeling in certain specific areas of the brain,and those selectively labeled cells in A8-9-10 all are dopaminergic neurons;c)these dopaminergi(?) cells are labeled through axonal uptake and retrograde transportof PI.
出处
《解剖学报》
CAS
CSCD
北大核心
1989年第1期27-33,共7页
Acta Anatomica Sinica
基金
国家自然科学基金
关键词
侧脑室
碘化丙啶
荧光标记
Fluorescent tracer
Propidium iodide
Lateral cerebral ventricle
Dopaminergic neurons
Purkinje cells
