摘要
目的 :探讨低氧对大鼠血清胰岛素样生长因子 -I(IGF -I)水平、肺小动脉壁IGF -I多肽的影响及其与低氧性肺动脉高压之间的关系。方法 :用图像分析技术检测肺小动脉的形态改变 ;RIA法检测血清IGF -I浓度 ;免疫组织化学染色法检测肺小动脉壁IGF -I多肽表达并进行定量分析。结果 :经 3周低氧后大鼠平均肺动脉压(mPAP)明显增高 (P <0 0 1) ;反映管壁增厚的两个指标MT %和MA %均显著高于正常对照组 (P <0 0 5 )。灰度扫描示低氧大鼠肺小动脉壁的灰度值显著强于正常对照组 (P <0 0 1)。低氧大鼠血清IGF -I水平明显高于正常对照组(P <0 0 5 ) ;大鼠血清IGF -I水平与mPAP呈显著正相关 (r=0 74 5 ,P <0 0 1)。结论 :在低氧情况下 ,肺小动脉壁IGF -I多肽产生显著增加 ;并且IGF -I不仅以自分泌和旁分泌的方式刺激血管平滑肌细胞增生肥厚 ,而且可能还存在内分泌机制。
AIM: To study changes in IGF-I concentration in serum, IGF-I polypeptide in the wall of pulmonary arterioles during hypoxia and the relationship between their changes and hypoxic pulmonary hypertension. METHODS: Morphological change in pulmonary arterioles was determined by image pattern analysis technique, the concentration of IGF-I in the rat serum was measured by radioimmunoassay. The expression of IGF-I polypeptide was observed by immunohistochemical staining on the walls of pulmonary arterioles, and analyzed quantitatively. RESULTS: After exposing hypoxia for 3 weeks, mPAP of rats was elevated(P<0 01), and the walls of pulmonary arterioles in the rats with hypoxic pulmonary hypertension were observed to be thickened with both MT% and MA% higher significantly than those of normal (P<0 05, respectively). The gray scale value of gray scale scanning in the pulmonary arterioles in the hypoxic rats was higher than that in the normal rats(P<0 01). The level of IGF-I in serum in the hypoxic rats was higher than that in the normal rats (P<0 05). The level of IGF-I in the rat serum was positively correlated with mPAP(r=0 745, P<0 01). CONCLUSION: Under the hypoxic condition, IGF-I polypeptide production increased in the walls of pulmonary arterioles. Results implied that IGF-I stimulates vascular smooth muscular cell proliferation not only in autocrine and paracrine manner, but also in the endocrine manner.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2001年第1期36-39,共4页
Chinese Journal of Pathophysiology
关键词
低氧
高血压
肺性
胰岛素样生长因子I
Anoxia
Hypertension, pulmonary
Insulin-like growth factor Ⅰ
