摘要
对Monacusspp.3的固体发酵生产MonacolinK的培养条件和培养基进行了优化.考察了物料厚度、起始含水量、发酵时间、起始pH值等发酵条件,以及大米培养基中添加附加碳源、氮源、磷酸盐等的影响.结果表明,固体发酵的培养条件为:物料(大米)厚度在4cm以下,起始pH值自然,培养温度32℃,相对湿度60%～70%,发酵时间为12d;经单因素和正交试验得适宜的发酵培养基为:大米100g,甘油0.2g,玉米浆0.15g,起始含水量50%.且以此优化条件固体发酵培养,MonacolinK产量稳定在1.4mg/g左右,最高产量为1.6mg/g.
The medium components and fermentation conditions for the Monacolin K production by Monacus spp.3 solid fermentation were studied. The operating factors, including the thickness of rice, initial water content, fermentation time, initial pH, appendant carbon and nitrogen source, and the concentration of K_2HPO_(4) were investigated. The optimal fermentation conditions were determined as follow: the thickness of materials 4.0 cm; natural initial pH; culture temperature, 32 ℃; relative humidity, 60～70%; and fermentation time, 12 days. The fermentation medium was optimized by single factors and orthogonal experiments: 100 g rice, (0.2 g) glycerine, 0.15 g corn steep liquor and 50% initial water content. The yield of Monacolin K was stable at about 1.4 mg/g with the highest of 1.6 mg/g under the optimized condition and medium.
基金
国家化学工程与技术重点实验室开放项目(编号:20001860)资助课题.
