摘要
目的:比较载脂蛋白E(ApolipoproteinE,ApoE)基因多态在帕金森病及阿尔茨海默病(Alzheimer'sdisease,AD)患者中的分布情况。方法:采用聚合酶链式反应-限制性片断长度多态性(Polymerasechainreactionandrestrictionfragmentlengthpolymorphism,PCR-RFLP)方法,在68例帕金森病患者、104例AD患者和110例正常人中观察ApoE基因多态的分布,并通过χ2检验和比值比(OR)对该多态与帕金森病、AD作相关分析。结果:ApoEε2/ε3/ε4多态的分布存在明显的AD患者与对照组间的差异(等位基因,χ2=24.94,df=2,P=0.0000;基因型,χ2=25.663,df=5,P=0.0001)。帕金森病患者与对照组间不存在ApoE基因多态各等位基因频率分布的差异(χ2=1.45,df=2,P=0.482)。关联分析亦未显示与帕金森病关联(OR=4.28,P>0.05)。ApoEε2/ε3/ε4多态的分布在帕金森病-AD组间差异存在显著性意义(等位基因,χ2=10.85,df=2,P=0.0044基因型,χ2=13.17,df=5,P=0.022)。结论:ApoEε2/ε3/ε4多态可能是AD的疾病易感因子,但与帕金森病的疾病易感性关系不大。ApoEε2/ε3/ε4多态在帕金森病、AD的发病风险中的作用不同。
AIM:To explore the distribution of apolipoprotein E(ApoE) gene polymorphism in patients with Parkinson's disease and Alzheimer's disease. METHODS:The distribution of ApoE gene polymorphisms was observed with the method of polymerase chain reaction and restriction fragment length polymorphism(PCR RFLP) in 68 patients with Parkinson's disease(PD group),104 with Alzheimer's disease(ADgroup) and 110 normal persons(control group),and correlation analysis of the polymorphism and Parkinson's disease and Alzheimer's disease was made with χ2 test and odds ratio. RESULTS:The distribution of ApoEε2/ε3/ε4 in the AD group was significantly different from that in the control group(allele:χ2=24.94,df=2,P=0.000 0;genotype:χ2=25.663,df=5,P=0.000 1).There were no significant differences between the PD group and the control group in the distribution of allelic gene frequency of ApoE gene(χ2=1.45,df=2,P=0.482).The analysis showed no correlation to Parkinson's disease(OR=4.28,P >0.05).There were significant differences in the distribution of ApoEε2/ε3/ε4 polymorphism between AD group and PD group(allele:χ2=10.85,df=2,P=0.004 4;genotype:χ2=13.17,df=5,P=0.022) CONCLUSION:ApoEε2/ε3/ε4 polymorphisms may be the susceptible factor of Alzheimer's disease,while is not closely related to the susceptibilities of Parkinson's disease.ApoEε2/ε3/ε4 polymorphisms play different role in the risk of episode of Parkinson's disease and Alzheimer's disease.
出处
《中国临床康复》
CSCD
2003年第31期4262-4263,共2页
Chinese Journal of Clinical Rehabilitation
