摘要
目的 研究乌鲁木齐市老年人群的性别、年龄以及所受教育程度这三种与ApoE相关危险因子与AD发病风险之间的关系。方法 应用PCR -RFLP等技术对 6 0例AD患者和 90例对照者的ApoE基因进行分型 ,分别计算两组ApoE各等位基因、基因型的频率 ,按照性别、年龄以及所受教育程度进行分组分析。结果 ⑴女性的ε4等位基因频率在AD和对照组间有显著性差异 (ε4 AD:ε4 Control=2 0 .97:5 .0 0 ,P <0 .0 1) ;⑵小于 75岁的AD患者ε4等位基因频率大大高于同龄对照组(P <0 .0 1) ,而≥ 75的AD患者ε4等位基因频率明显低于 75岁以下AD患者 (P <0 .0 1) ;⑶文盲和小学文化程度的AD患者的ε4等位基因频率明显高于对照组 ,而高中文化程度的AD患者其ε4等位基因频率却低于对照 ,统计学处理说明这些差异都有显著性 (P <0 .0 1)。结论 :⑴携带ε4等位基因的女性AD发病风险高于男性 ;⑵ 75岁以下ε4携带者具有高的AD发病风险 ;⑶受教育程度低且携带ε4等位基因者易患AD。
Objective: To analyse the relation between three risk factors (gender, age, and education degree) which are associated with ApoE and AD. Methods: PCR-RFLP techniques were used to count the distribution of genotype,gene frequency of ApoE alleles in 60 cases with AD and 90 normal as control. Results ⑴The ε4 allelic gene frequency of female between AD and control was significantly different (ε4 AD:ε 4 Control = 20.97:5.00,P<0.01); ⑵There was a higher ε4 allelic gene frequency in AD than in control in the groups of <75 year old(P<0.01). However,the ε4 allelic gene frequency of in the groups of ≥75 year old was lower than that of the group of <75 year old in ADP0.01); ⑶There was a higher ε 4 allelic gene frequency in AD than in control in the groups of illiteracy and primary school level,however, the ε 4 allelic gene frequency in AD was lower than in control in the groups of senior high school level, and the statistical treatment suggest that there are both significant differences(P<0.01). Conclusion ⑴The carrier of ε4 of female has higher developing AD risk than that of male; ⑵The carrier of ε4 with age of <75-year-old is vulnerable to AD; ⑶The carrier of ε 4 with lower education level has a higher risk in developing AD.
出处
《中国优生与遗传杂志》
2004年第5期36-37,28,共3页
Chinese Journal of Birth Health & Heredity
