摘要
目的:脊髓缺血再灌注可使组织自身产生炎症反应,中性粒细胞(PMNL)的浸润加重组织损伤。观察缺血再灌注脊髓组织中24h内不同阶段PMNL的黏附、聚集及浸润情况。方法:采用腰动脉阻断法建立兔脊髓缺血再灌注损伤模型,分别缺血40,60min后再灌注,对不同时间段损伤脊髓节段行光镜观察。结果:在缺血期均未观察到PMNL的黏附、浸润,随着再灌注的进程,病理改变的加重,组织中PMNL数量逐渐增多;6h时PMNL主要黏附、聚集在血管中,甚至堵塞血管腔部;同时可见其穿透血管壁向外游出,实质中可见少量的PMNL浸润;12,24h时大量PMNL浸润在实质中,并形成“噬神经元”现象。此外,不同的缺血时间,其各自再灌注后PMNL聚集、浸润程度不同;在同一时间点上,缺血时间长、损伤重的,PMNL出现数量多。结论:脊髓缺血再灌注早期(24h内),PMNL最初聚集并阻塞血管,然后浸润到组织实质中,从而加重损伤;进一步阐明中性粒细胞参与脊髓缺血再灌注损伤的作用过程。
AIM: Ischemia/reperfusion can caused self inflammatory reaction in the spinal cord tissues, the infiltration of polymorphonuclear leukocyte (PMNL)can exacerbate the tissue damage. To observe the adherence, aggregation and infiltration in spinal cord tissues during 24h ischemia/reperfusion period. METHODS: The models of ischemical reperfusion injury in the spinal cord of rabbit were made by using the occluding lumbar arteries method, they were reperfused after ischemia for 40 min and 60 min, respectively.The damage in segment of spinal cord of different period were observed with light microscope. RESULTS: The adherence, aggregation and infiltration in PMNL couldn't be observed during the ischemia period. Following reperfusion and histopathologic exacerbation and, the gradual increase of the PMNL in spinal cord tissues occurred along with the process of reperfusion.The adherence and aggregation at the 6 h were mainly in the blood vessel,or even blocked in the lumen of blood vessel, the emigration outward of them through the vascular wall could be observed at the same time, and also a few infiltration of PMNL in parenchyma. There were a lot of infiltration of PMNL in parenchyma at the 12 h and 24 h, and occurred the phenomenon of neuronphage.Besides, the level of aggregation and infiltration of PMNL after reperfusion at different ischemia period.At the same time point, the longer the ischemia period and the severer the damage was, the more the PMNL were. CONCLUSION:During the early period(within 24 h) of ischemia/reperfusion in spinal cord, the PMNL aggregates and blocks the blood vessel at first, then infiltrates into the parenchyma of the tissues, so as to exacerbates the damage. It explains further that the PMNL takes part in the process of ischemia/reperfusion damage in spinal cord.
出处
《中国临床康复》
CSCD
2003年第31期4192-4193,T001,共3页
Chinese Journal of Clinical Rehabilitation
基金
苏州大学附属第一医院课题资助项目(H0304)~~
