摘要
作者在1976年发现所有植物各部位中均含有可能是一种水解酶(暂定名为水解酶Ch),它能水解2,6-二氯乙酰靛酚,使水溶液由浅黄色变为深蓝色.酶活力能被有机磷农药抑制,但不能水解溴化乙酰胆硷.本文研究了水解酶Ch在植物各部位中的分布情况,发现酶活力大小顺序为;种子>茎>根;新根>旧根>败叶>叶,块根>通常根,即更多地存在于植物生命力旺盛的部位,它可能在植物生理学方面起重要作用.
The authors observed in 1976 that all parts of plants contained the eazyme,hydrolase Ch,which catalytically hydrolyzed the substrate 2,6-dichlorophenol indophenol acetate,changing its color from light yellow to deep blue in aqueous solution.Like cholinesterase,the hydrolytic activity of hydrolase Ch may be inhibited by organic phosphorus compounds,but it differs from cholinesterase in that it does not catalytically hydrolyze acetylcholine bromide at pH 7-8,In this report,the distribution of hydrolase Ch in different parts of plants was investigated.The activities of the enzyme in plants are in the following orders:seeds>leaves>stems>roots;new roots>old roots;dead leaves>growing leaves.More hydrolase Ch was found in the growing regions than in other parts and plays an important role in reproductive and vegetative development in plants.The velocities of recovery of activities inhibited by different pesticides were different.The methods may be used as a way to assess the quality of pesticides.
出处
《军事医学科学院院刊》
CSCD
北大核心
1989年第3期214-217,共4页
Bulletin of the Academy of Military Medical Sciences
关键词
水解酶
CH
二氯乙酰靛酚
hydrolase Ch
2,6-dichlorophenolindophenol acetate
