摘要
目的 建立血清p185蛋白新的检测方法，评价血清p185蛋白在乳腺癌诊断中的意义．方法 利用生物素化CerbB-2单克隆抗体，建立血清p185蛋白BA—ELISA检测方法，并对乳腺癌、乳腺增生和正常对照血清进行p185蛋白检测，同时对组织进行p185免疫组化染色。结果 乳腺癌血清p185蛋白与乳腺增生、正常时照相比差异显著(P<0.05)，其中免疫组化阳性乳腺癌血清p185蛋白与乳腺增生、正常对照组相比有显著差异(P<0．01)，而免疫组化阴性乳腺癌血清p185蛋白与其相比无显著差异(P>0．05)；BA-ELISA方法检测血清p185蛋白的敏感度为43.3％，特异度为91．1％，准确度为74．4％、血清p185蛋白水平与乳腺组织p185蛋白表达高度相关(P<0．01).结论 乳腺癌患者血清p185蛋白对乳腺癌具有诊断意义．血清p185蛋白与组织p185蛋白表达高度相关.
Objective To establish a new method for seral p185 and investigate clinical significances of seral p185 for breast cancer. Methods Using bio-C-erbB-2 monoclonal antibody, BA-ELISA for seral p185 was established and seral p185 was detected in breast cancer, breast proliferation patients and normal control. The expression of p185 was detected in breast tissues with immunohistochemical assay.Results There are obvious differences comparing breast cancer group with breast proliferation group and normal control group in p185 level(P<(0.05)). In positive p185 staining, there are significant differences comparing the breast carcinoma group with breast proliferation group and normal control group(P<(0.01)). In negative p185 staining, there is no significant differences comparing the breast cancer group with breast proliferation group and normal control group in seral p185 levels(P>(0.05)).The sensitivity,specificity and accuracy of BA-ELISA for seral p185 is (43.3%), (91.1%) and (74.4%) respectively. There is obvious relationship between seral p185 level and tissue's p185 expression(P<(0.01)).Conclusion Seral p185 is of clinical significance in diagnosing breast carcinoma. There is obvious relationship between seral p185 level and tissue's p185 expression in patients with breast carcinoma.
出处
《北华大学学报（自然科学版）》
CAS
2004年第1期27-30,共4页
Journal of Beihua University（Natural Science）
基金
吉林省卫生厅科学基金资助项目(2001)
