摘要
目的:获得TRAIL全长基因并在原核表达系统中表达。方法:通过RT-PCR方法,从人外周血细胞中克隆TRAIL全长基因,再克隆其至原核表达载体pGEX-2T中,并在大肠杆菌JM109细胞中表达。结果:获得了TRAIL基因。序列分析表明,该序列与GenBank数据库中的序列一致。其原核表达产物能够抑制肿瘤细胞系KB细胞及Hela细胞的生长,最终导致细胞凋亡。结论:原核表达体系表达了具有生物学活性的TRAIL,为进一步研究其抗肿瘤活性奠定了基础。
AIM: To obtain and express the full-length of TRAIL gene in prokaryocytes. METHODS: The full-length of TRAIL gene was amplified from peripheral blood cells(PBCs)by RT-PCR and then cloned into the expression vector pGEX-2T. The TRAIL-GST was expressed in E. coli. RESULTS: Sequence analysis indicated that the sequence of TRAIL gene was identical with that in the GenBank. The TRAIL-GST was expressed in E. coli. Expressed product could inhibit the proliferation of KB and Hela cells and finally lead to their apopto-sis. CONCLUSION: The TRAIL-GST with biological activity has been expressed in the eukaryotic system.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2003年第6期535-537,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
军队"九五"课题基金资助(No.96M174
98z089)
