摘要
目的为建立环境雌激素基因重组酵母测评系统,构建雌激素效应元件(estrogenresponseelements,ERE)调控的酵母报告载体。方法人工合成ERE寡核苷酸片段,先与经SalI酶切和CIAP去磷酸化处理的pLacZi连接成pLacZi-1ERE,再与经XhoI酶切和CIAP去磷酸化处理的pLacZi-1ERE连接,得到pLacZi-2ERE。结果序列分析结果表明,构建成功2个串联拷贝ERE调控的酵母报告载体。结论该文构建成功ERE调控的酵母报告载体,为进一步建立环境雌激素基因重组酵母测评系统奠定了基础。
Objective To establish the recombinant yeast estrogen system(RYES),a reporter vector controlled by estro-gen response elements(ERE)in yeast was constructed.Methods pLacZi-1RER was obtained first by inserting an ERE which was synthesized artificially into the yeast reporter vector pLacZi which had already been lined by SalI and the terminal dephos-phorized by CIAP.Then,pLacZi-zERE.was obtained by inserting another ERE into the XhoI site nearby the ERE in pLacZi-1ERE.Results Sequence analysis indicated that the yeast reporter vector controlled by two tandem copies of ERE was con-structed successfully.Conclusion The yeast reporter vector controlled by ERE,pLacZi-2ERE,was constructed successfully,which was the very important groundwork for further establishment of the recombinant yeast estrogen system(RYES).
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2003年第1期39-41,共3页
Journal of Environment and Health
基金
国家自然科学基金重点资助项目(30030120)
