摘要
目的 :研究大鼠周围神经损伤与再生过程中生长相关蛋白的表达。方法 :取正常SD大鼠坐骨神经和离断损伤后不同时间近侧端和远侧端神经组织 ,采用Anti GAP 43抗体以WesternBlot方法检测GAP 43的表达变化。结果 :NC膜上 43kDa位置出现阳性反应条带 ,在正常坐骨神经中反应微弱 ,损伤后明显加强 ,随损伤时间延长 ,神经近侧端的反应无明显改变 ,神经远侧端的反应逐渐加强 ,以第 3周为最强 ,之后逐渐减弱。结论 :GAP 43在正常坐骨神经中低表达 ,损伤后近侧端与远侧端表达均增加 ,远侧端的表达增加以第 3周为高峰。
Objective: To study the expression of growth associated protein 43(GAP 43) in nerve regeneration of rat with injured sciatic nerve.Methods:By Anti GAP 43,Western Blot was used to examine the different expression of GAP 43 in the distal and proximal stump of post transected sciatic nerve and nerve of SD rat.Results: The positive band was found around 43kDa on NC membrane,the reaction was weaker in the intact sciatic nerve and deeper significantly after nerve injury.Following injury time,there was no much change in the proximal stump,positive reaction in the distal stump increased to the deepest at the third week,then it was decreased.Conclusion:The expression of GAP 43 was in the low levels and increased significantly after sciatic nerve injury,and the expression reached the highest levels at the third week in the distal stump.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2003年第1期55-57,共3页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金资助项目 (30 0 70 2 55)
