摘要
目的:研究不同浓度白藜芦醇(Res)及与环孢霉素A(CsA)联用时,对人外周血T细胞(hPBTC)的增殖、淋巴细胞母细胞化及IL-2、INF-γ产生的影响。方法:采用MTT比色法检测hPBTC的增殖。形态学方法观测T细胞转化率。ELISA法检测淋巴细胞培养上清液中IL-2、INF-γ含量。结果::Res的浓度>2.5 mg/L时,hPBTC增殖及淋巴细胞的转化率均明显降 低(P<0.05),以10 mg/L的效果最为显著;Res浓度>5 mg/L时,培养上清液中IL-2和INF-γ的含量下降明显(P<0.05)。Res浓度>2.5 mg/L时与CsA联用具有协同作用。结论:Res对人淋巴细胞的增殖及母细胞化具有明显的抑制作用,在一定的浓度范围内其与CsA联合应用能增强免疫抑制作用。
AIM: To investigate the effect of resveratrol (Res) alone and its combination with cyclosporin A (CsA) on the proliferation of human peripheral blood T lymphocytes (hPBTCs), transformation into lymphoblasts, as well as IL-2 and INF-y production. METHODS: The proliferation of hPBTCs was detected by MTT colorimetry. The lymphocyte transformation rate was examined by morphologic method. The levels of IL-2 and INF-y in lymphocyte culture supernatant were detected by ELISA. RESULTS: When the concentration of resveratrol was over 2. 5 mg/L, especially 10 mg/L, the proliferation and transformation rate of hPBTCs decreased significantly ( P < 0. 05). The levels of IL-2 and INF-y in lymphocyte culture supernatant reduced markedly (P<0.05), on condition that the concentration of resveratrol was over 5 mg/L. The combination of more than 2. 5 mg/L of resveratrol with cyclosporine A had synergism. CONCLUSION: Resveratrol can suppress notably the proliferation and transformation of human lymphocytes and the combination of resveratrol at a given concentration with cyclosporine A can enhance immune suppression.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2003年第6期549-551,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
陕西省自然科学基金资助项目(No.2002C249)
