摘要
目的测定复方抗病毒制剂体外对流感病毒和呼吸道合胞病毒的抗病毒作用。方法用鸡红细胞血凝素滴定法测定不同浓度复方抗病毒制剂在狗肾传代细胞(MDCK)中对流感病毒的抑制作用;用中和实验测定复方抗病毒制剂在Hep-2细胞中对呼吸道合胞病毒的活性。结果复方抗病毒制剂浓度<1.5 mg.mL-1时对MDCK细胞及Hep-2细胞无明显毒性。浓度>0.15 mg.mL-1能抑制流感病毒的血凝活性和呼吸道合胞病毒对靶细胞的攻击作用。结论复方抗病毒制剂体外对流感病毒和呼吸道合胞病毒具有较强的抑制作用,且有较大的安全性。
Objective To investigate the antivirus activity of the antiviral herb compound on influenza virus and respiratory syncytial virus（RSV）. Methods The suppression on influenza virus by different doses of antiviral herb compound in MDCK passage cells was evaluated by hemaglutinin titration test, while the effect on RSV was studied by the neutralization test in Hep-2 cells. Results No obvious toxic effects on MDCK cells and Hep-2 cells were observed by the compound with the concentration lower than 1.5 mg·mL^-1. However, it suppressed the hemagglutination of influenza virus and the aggression of RSV on target cells at the concentration over 0. 15 mg·mL^-1. Conclusion The antiviral herb compound shows potent inhibition on the influenza virus and RSV in vitro with a high safety.
出处
《医药导报》
CAS
2008年第4期392-394,共3页
Herald of Medicine
关键词
抗病毒制剂
复方
病毒
流感
合胞病毒
呼吸道
体外活性
Antiviral herb compound
Influenza virus
Respiratory syncytial virus
Antiviral activity
