摘要
目的 建立反相高效液相色谱法 (RP HPLC)测定原料药及注射剂中丹皮酚 (paeonol,Pae)的含量。 方法 采用LichrospherC18柱 (4 6mm× 2 5 0mm ,粒径 5 μm) ;以甲醇乙腈水 (30∶4 0∶30 )为流动相 ;流速为 0 8ml·min-1;检测波长为 2 74nm ;柱温为 2 5℃ ;进样量为 2 0 μl。结果 Pae的线性范围为 0 0 2～ 2 5 6 0mg·L-1,回归方程为 ^Y =3772 5 2 5 8X - 0 74 74 ,r =1 0 0 0 0 ,n =5 ;平均回收率为99 87% ;日内、日间变异均小于 4 %。结论 该法简便、快速 ,可用于Pae及其制剂的含量测定。
AIM To determine the concentration of paeonol in the bulk drug and injection, a method of reversed-phase high performance liquid chromatography (RP-HPLC) was developed. METHODS Lichrospher C_ 18 column(4.6 mm×250 mm, 5 μm)was used with methanol-acetonitrile-water(30∶40∶30)as mobile phase at a flow rate of 0.8 ml·min -1 . 20 μl of sample was injected into the C_ 18 column where the temperature was 25℃. The detection wavelength was 274 nm. RESULTS The linear regression equation for paeonol was = 3 772.525 8 X- 0.747 4 (r = 1.000 0, n =5) under the linearity range from 0.02 mg·L -1 to 25.60 mg·L -1 . The average recovery was 99.87%. The relative standard deviations of the intra-day and inter-day were less than 4%. CONCLUSION The method is simple and rapid, which may be used for the determination of paeonol and its preparation for different purpose.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2003年第5期593-595,共3页
Chinese Pharmacological Bulletin
基金
安徽省自然科学基金资助课题
No 0 0 0 44 414
