摘要
目的:观察糖尿病大鼠脊髓神经元型一氧化氮合酶(nNOS)免疫阳性神经元数量的变化,探讨NO在糖尿病发生和发展中的作用机制。方法:用链脲佐菌素诱导建立糖尿病大鼠模型,ABC免疫细胞化学法显示nNOS免疫阳性神经元。结果:大鼠脊髓内nNOS阳性神经元主要分布于中央管周围灰质和中间带等区域。中间带外侧核可见nNOS免疫阳性神经元较集中,细胞突起呈束状伸向中央管周围灰质方向;定量分析显示,糖尿病大鼠脊髓中央管周围灰质和中间外侧核在7 w、12 w时nNOS免疫阳性神经元数量明显增多。结论:糖尿病时伤害性刺激的传入增多,增多的nNOS免疫阳性神经元可能与痛觉过敏等糖尿病周围神经病变有关。
Objective : To discuss the role of nitric oxide in the development of diabetes mellitus by observing the numerical changes of neuronal nitric oxide synthase immunoreactive (nNOS-ir) neurons in spinal cord of diabetic rats. Methods : Diabetic rats was induced by intraperitoneal administration of streptozotocin (STZ). The method of ABC immunocytoche-mistry was used to show the nNOS-ir neurons. Results : nNOS-ir neurons appeared in the central grey matter and the lateral nucleus of spinal cord. nNOS-ir neurons concentrated in the lateral nucleus, and the processes of neurons bunchily extended to the central grey matter. The quantitative analysis showed that the number of nNOS-ir neurons around the central grey matter and the lateral nucleus in spinal cord of diabetic rats was significantly increased in 7 and 12 weeks. Conclusion : The afferents from noxious stimuli increase in diabetic rats; the increase of nNOS-ir neurons maybe related to diabetic peripheral neuropathy like hyperalgesia.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2003年第5期439-442,共4页
Chinese Journal of Anatomy
基金
广东省中医药管理局科研基金(100068
401040)
