摘要
目的 观察高脂血症大鼠海马中神经元型一氧化氮合酶 (nNOS)及神经营养因子 3(NT 3)的变化 ,并探讨其临床意义。方法 采用HE和SABC免疫组织化学染色方法 ,结合计算机图像分析系统 ,比较两组海马各区平均光密度(MOD)值。结果 与对照组相比 ,实验组血清总胆固醇(6 6 5mmol/L)及血清低密度脂蛋白胆固醇 (4 4 1mmol/L)升高明显 ,差异有显著性。实验组海马各区nNOS免疫阳性神经元MOD升高 ,尤以CA3区明显 ;NT 3免疫阳性神经元MOD降低 ,以CA1区最为显著。结论 高脂血症大鼠海马中nNOS表达增强而NT 3表达减弱 ,表明高脂血症可导致海马神经元功能改变。
Objective To observe the changes of nNOS and NT-3 in hippocampal neurons of hyperlipidemic rats, and investigate their clinical significance. Methods HE and SABC immunohistochemistry staining was used. And the optic density of nNOS and NT-3 immunoreactive neurons in hippocampus was detected by computerized image pattern analysis. Results Compared with control group, experimental group serum TC and LDLC increased to 6.65 mmol/L and 4.41 mmol/L, and mean optical density level of nNOS and NT-3 immunoreactive neurons in rats hippocampus increased and decreased respectively. All the differences were significant. Conclusion In hippocampus of hyperlipidemic rats the expression of nNOS increased but that of NT-3 decreased suggesting that hyperlipidemia can cause and functional changes of neurons in hippocampus.
出处
《安徽医科大学学报》
CAS
2004年第3期177-181,共5页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金资助项目(编号:39870324)
安徽省自然科学研究基金资助项目(编号:99044312)
安徽省教育厅自然科学基金资助项目(编号:2001kj123)
关键词
高脂血症
一氧化氮合酶
神经生长因子
免疫组织化学
海马
大鼠
hyperlipidemia
nitric oxide synthase
nerve growth factors
immunohistochemistry
hippocampus
rats
