摘要
目的:观察正常成年猕猴背根神经节神经元型一氧化氮合酶(nNOS)免疫阳性神经元的分布。方法:ABC免疫细胞化学方法显示nNOS免疫阳性神经元,并用体视方法进行定量分析。结果:猕猴颈、胸、腰各段背根神经节nNOS免疫阳性神经元的分布相似,数量较多,阳性神经元的大小不等,多呈圆形或椭圆形;胞浆着色较深,胞核位于细胞中央,不着色,细胞被神经纤维束分隔成群。nNOS免疫阳性神经元以中型神经元为主,其次为小型神经元,其胞浆呈强阳性染色,细胞直径<50μm,大型神经元较少。颈、胸、腰各段背根神经节nNOS免疫阳性神经元的密度以及阳性细胞与总细胞数的比值均无明显差异。结论:猕猴背根神经节nNOS主要表达在中、小型神经元,提示NO可能主要参与痛觉等浅感觉的传导和调制。
Objective:To study the distribution of neural nitric oxide synthase (nNOS)-immunopositive neurons in dorsal root ganglions (DRGs) in rhesus macaques. Methods: After cryostat section, ABC immunohistochemitiy method was used to detect the nNOS-immunopositive neurons in DRGs,and stereology method was used for quantitative analysis. Results: In DRGs, there were a lot of clearly displayed nNOS-immunopositive nerons, and the distribution of them in different spots of DRGs was similar to others. The sizes of them were different,and their shapes were round or ellipse. The cytoplasm was deeply pigmented, and nucleus lay in the center of neuron without pigmentation. The immunopositive neurons were partitioned by nerve fiber bundles. Most of immunopositive neurons were medium-sized, then were the small-sized, they all displayed strong staining (neuron diameter< 50 um), the large-sized immunopositive neurons were less than the small-sized and medium-sized cells. Statistics analysis showed the density of nNOS-immunopositive nerons had no significant different (P > 0.05), and the rate between immunopositive nerons and sum of neurons had the similar results (P > 0.05). Conclusion: In DRGs of rhesus macaques,nNOS mainly exists in the medium-sized and small-sized neurons,indicating that nitric oxide (NO) may chiefly take part in the modulation of superficial sensation such as the pain,efc.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2003年第1期30-34,共5页
Chinese Journal of Anatomy
