摘要
利用组织学连续石腊切片的研究方法,观察了糜子组织培养中植株再生的过程,从而证明了植株再生是通过体细胞胚胎发生途径的。结果表明:(1) 糜子成熟胚培养首先从下胚轴及胚根区愈伤组织化;(2) 体细胞胚起源于下胚轴及胚根维管束周围愈伤组织中单个离散的胚性细胞;(3) 糜子离体体细胞胚有与典型禾谷类作物合子胚大致相似的发育过程。
The histology of somatic embryogenesis of Panicum miliaceum in vitro is described here. The mature embryos produced embryogenic callus and the callus regenerated plants. The results of histological observation indicated that (1)callus was first formed from the subepidermal ceils near the hypocotyl and especially the radicle;(2) somatic embryogenesis was initiated from the isolated single embryogenie cells in the loose callus near the vascularity of the hypocotyl and radicle;(3) there were quite similarities in development between the somatic embryos of P.miliaceum and the zygotic embryos of typical cereal crops.
出处
《西北植物学报》
CAS
CSCD
北大核心
1992年第1期17-21,共5页
Acta Botanica Boreali-Occidentalia Sinica
关键词
糜子
胚培养
组织学
Panicum miliaceum L., embryo culture, plant regeneration, somatic embryogenesis, histology.
