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小麦慢叶锈性品种与叶锈菌相互作用的组织学研究 被引量:3

The Histological Study on The Interaction of Slow Leaf Rusting Wheat Cultivars and Puccinia recondita f.sp.tritici
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摘要 本文研究了黑芒麦、宜安52选、平原50、阿夫、冀麦3号和尤皮2号六个小麦品种与叶锈菌360、376、60、61四个小种的相互作用。通过对叶片透明染色,对病原菌从侵入、扩展到形成孢子的整个过程都作了组织学观察及统计分析。结果表明,慢锈品种与感病品种相比,在抗侵入方面并无差异,而在对菌丝体扩展、吸器的形成上慢锈品种的挪制作用显著大于感病品种。观察发现,在慢锈品种中同样存在着过敏性组织坏死,这可能是导致慢锈性的机制之一。通过分析认为,菌丝体扩展面积、吸器形成的数量以及坏死组织的面积,可作为评价慢锈性的生物学指标。在对菌丝体扩展面积和孢子堆面积的方差分析中表明,无论在品种间、小种间、还是在二者的互作间,均存在着显著的差异,因此慢锈品种也有专化性,也有抗性“丧失”的可能,应引起足够的重视。 The interaction between six wheat cultivars and four physiologic races of Puccinia recondita f.sp.tritici has been studied histologically. By means of a Whale leaf Clearing and staining technique,observation and statistical analysis have been made on studying the whole cource of develop- ment of the pathogens from their penetration to the formation of uredio- spores.The result showed there was no statistical difference between the slow-rusting cultivars and susceptible cultivar in the penetration of the pathogens,but the effect of inhibition to the development and the forma- tion of haustoria in the slow-rusting eultivars was much greater than that on the susceptible cultivar.Hypersensitive reaction of the host has been ibserved in some of the slow-rusting cultivars and this may contribute to one of the mechanisms of resistance.The authors think that the area of hyphae extention,the number of haustoria and the area of necrosis may be used as biologic criterion in evaluating the resistance of slow-rusting culti- vats.The analysis of variance on the areas of hyphae extention and the area of pustule size showed that differences were significant either between cultivars,races or their interactions.These specificities warrant that slow rusting cultivars still may have the possibility of lossing their resist- ance if encountering races with different virulence.
出处 《河北农业大学学报》 CAS CSCD 北大核心 1989年第3期91-98,共8页 Journal of Hebei Agricultural University
关键词 小麦 叶锈病 组织学 Wheat Leaf rust Histology
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  • 1曹克强,王焕如.小麦品种慢叶锈性及其变异性和专化性研究[J].植物病理学报,1989,19(1):53-58. 被引量:4
  • 2Komer J A. Genetics of resistance to wheat leaf rust. Annual Review of Phytopathology, 1996, 34:435 -455.
  • 3Kloppers F J, Pretorius Z A. Effects of combinations amongst genes Lr13, Lr34 and Lr37 on components of resistance in wheat to leaf rust. Plant Pathology, 1997, 46 : 737 - 750.
  • 4Hu G G, Rijkenberg F H J. Scanning electron microscopy of early infection structure formation by Puccinia reconditat f. sp. tritici on and in susceptible and resistant wheat lines. Mycological Research, 1998, 102(4):391-399.
  • 5Southerton S G, Deverall B J. Changes in phenylalanine ammonia-lyase and peroxidase activities in wheat eultivars expressing resistance to the leaf-rust fungus. Plant Pathology, 1990, 39 ( 2 ) : 223 - 230.
  • 6Zhang L, Meakin H, Dickinson M. Isolation of genes expressed during compatible interactions between leaf rust ( Puccinia triticina) and wheat using cDNA-AFLP. Molecular Plant Pathology, 2003, 4(6) : 469 -477.
  • 7Niks R E. Comparative histology of partial resistance and the nonhost reaction to leaf rust pathogens in barley and wheat seedlings. Phytopathology, 1983, 73(1 ) : 60 -64.
  • 8Jacobs T H, Buurlage M B. Growth of wheat leaf rust colonies in susceptible and partially resistant spring wheats. Euphytica, 1990, 45(1) : 71 -80.
  • 9Jacobs T. Abortion of infection structures of wheat leaf rust in susceptible and partially resistant wheat genotypes. Euphytica, 1990, 45(1) : 81 -86.
  • 10Southerton S G, Deverall B J. Histological studies of the expression of the Lr9, Lr20 and Lr28 alleles for resistance to leaf rust in wheat. Plant Pathology, 1989, 38:190 -199.

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